Accelerate Bioprocessing.
Reagents designed to harness the powers of affinity and phase separation to improve the manufacturability, purity & quality of advanced therapeutics.
Achieving high LV yields and purity with our chromatography-free process
Associate Director of R&D for Isolere Bio, Nikki Votaw, PhD, on innovative approaches to lentiviral vector purification, achieving high yields, and purity with a chromatography-free process. View Webinar→
Breaking free from Chromatography:
Transformative AAV Purification
Director of Advanced R&D, Michael Dzuricky, Ph.D., on how IsoTag™ AAV Reagent can achieve >90% AAV capture and 60-85% elution recoveries with our baseline protocol on the first run. View Webinar→
IsoTag™ AAV
Reagent
10mL Evaluation Kit
Scalable, chromatography-free purification with high purity and yield in under 4 hours for a broad spectrum of AAV serotypes.
IsoTag™ LV
Reagent
5mL Reagent
Scalable, chromatography-free purification with high purity and yield of VSV-G pseudotyped vectors in under 8 hours.
Rapid processing, high purity,
and the ability to consolidate unit operations
IsoTag™ Reagent Technology
Reagents with Affinity and Phase Separation
Phase separation is a phenomenon evolved by cells over billions of years to perform highly specific separations in complex environments. We are developing reagents that harness this same power — they combine precisely tunable phase separation with affinity ligands designed to have exquisite specificity for their target biologic.
Rapidly Bind in Solution
The binding domain targets a specific biomolecule of interest (i.e. protein, virus, nucleic acid) and the polypeptide confers its phase separation capability to said target. This process occurs in solution, in seconds, circumventing any mass transfer limitations.
Concentrate & Wash
Via affinity, the unique material properties of our reagents can be conferred to the biologic target of interest to pull them into large droplets that are a separate phase from otherwise aqueous contaminants.
Dissociate & Collect Final Product
Our reagents are then paired with high-performing filtration processes that use the size of the droplets to streamline purification. Our reagents have also demonstrated stabilizing and protective effects, yielding higher quality final product at higher step yields.
Life Science evolves.
Donaldson solves.
Isolere Bio is part of Donaldson and its focus on Life Sciences and Bioprocessing. Learn more 🡒
We are proud to collaborate with:
