Concentrate and purify AAV in under 4 hours using standard TFF equipment.
Achieve high yields with minimal optimization.
Available for a Limited Time Only!
This product is Prototype Research Grade and currently limited to customers in the US and Canada. For international shipping outside of North America, please contact us here.
Perform continuous capture in a fed-batch format to concentrate your material. Perform a series of wash step to eliminate host cell contaminants. Achieve your desired purity through predictable contaminant removal as a function of the number of wash diavolumes programmed.
The purity of AAV product achieved with IsoTag™ reagent is superior to industry leading methods, as assessed by CE-SDS. Aggregates are also lower in the IsoTag™ product compared to that from leading affinity chromatography resins (as measured with many techniques, including DLS, SEC, and CD-MS).
IsoTag™ AAV is a specialized reagent, engineered for the demanding requirements of small and large-scale downstream purification. It combines the principles of affinity capture with liquid-liquid phase separation using a proprietary fusion protein.
The single protein reagent has two domains: (1) an AAV-specific binding domain and (2) a stimulus-responsive biopolymer. It enables a robust, efficient, and consistent purification process for a broad spectrum of adeno-associated virus (AAV) serotypes.
The IsoTag™ AAV Reagent Kit is for research use only and includes pre-made buffer solutions. It is currently limited to customers in the US and Canada. For international shipping outside of North America, please contact us here.
IsoTag™ reagents are shipped on dry-ice via expedited services and require immediate storage at -80°C upon receipt.
Achieve 95% or greater AAV capture and 60-85% elution recoveries with our baseline protocol on the first run.
We’ll work with you to continue optimizing a protocol that can achieve the benchmarks you set for your process and pipeline.
Concentrate, wash, and elute in a single TFF step. Open pore sizes facilitate rapid processing with high flux and stable TMPs. Our process is compatible with standard TFF equipment and validated, off-the-shelf filters, making it easy to plug into your existing downstream process.
IsoTag™ AAV Reagent Kit
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