Concentrate and purify AAV in under 4 hours.
Achieve high yields & scale-up linearly with minimal optimization.
Breaking free from Chromatography:
Transformative AAV Purification
Director of Advanced R&D, Michael Dzuricky, Ph.D., on how IsoTag™ AAV Reagent can achieve >90% AAV capture and 60-85% elution recoveries with our baseline protocol on the first run. View Webinar→
Linear scalability of IsoTag™ AAV Affinity Liquid Phase Separation TFF Process (PDF)
This study highlights the simplicity of scaling the ALPS-TFF process by using consistent parameters – leading to predictive performance across volumetric scales. The linear scalability of IsoTag™ AAV TFF processes offers the potential to streamline the process development required to scale affinity purification of AAV. Download the full Application Note ⇲
IsoTag™ AAV Reagent
Streamlined Adeno-associated Virus Purification without Chromatography
IsoTag™ AAV is a specialized reagent, engineered for the challenging requirements of small and large-scale manufacturing. It enables a robust, efficient, and consistent downstream purification process for a broad spectrum of adeno-associated virus (AAV) serotypes. Purification with IsoTag AAV does not require the use of chromatography processes nor column packing, and is designed to reduce the costs and challenges associated with scaling from R&D to commercial production.
Bind in solution
The binding domain targets a specific biomolecule of interest, binding in seconds (no mass transfer limits)
Concentrate & wash
Pull target into large droplets that are in a separate phase from other aqueous contaminants.
Final product
Efficiently collect high-yield product using existing equipment and off-the-shelf consumables.
Results from the centrifugation (ALPS-CF) buffer screen directionally translate to the scalable tangential flow filtration (ALPS-TFF) process. IsoTag™ AAV Reagent is paired with platform processes that use existing equipment and off-the-shelf consumables.
ALPS-CF small scale: 1mL to 50mL
Use with microcentrifuge tube, 50mL tube and beyond with low-speed (5000 x g) centrifuge
ALPS-TFF scalable: volumes > 1L
Use with existing TFF equipment and hollow fiber filters
+ Scalability
ALPS-TFF scales linearly, increasing filter surface area with harvest volume
+ Yield
>90% capture and 75% elution of both total virus capsids
+ Quality
≥4 LRV of contaminating host cell proteins & DNA in harsh conditions
IsoTag™ reagents are paired with platform processes that use existing equipment and off-the-shelf consumables to achieve high purity and yield in hours. IsoTag AAV can utilize a microfiltration hollow fiber and, as such, is run in permeate control mode to maintain a constant flux throughout the process. This allows completion of the process in under 4 hours regardless of scale & can consolidate multiple unit operations, into one-step TFF.
Improved yields
High product quality
No size target limitations
A variety of AAV serotypes demonstrated binding efficacy in small-scale capture tests
(note: elution may require optimization)
IsoTag™ AAV Reagent
This evaluation kit contains IsoTag™ AAV reagent and the buffers required to purify approximately 1.5 liters of clarified AAV harvest material in a TFF process. This product is currently limited to customers in the US and Canada. For international shipping outside of North America, please contact us.
Breaking free from Chromatography:
Transformative AAV Purification
Director of Advanced R&D, Michael Dzuricky, Ph.D., on how IsoTag™ AAV Reagent can achieve >90% AAV capture and 60-85% elution recoveries with our baseline protocol on the first run. View Webinar→
More on: IsoTag™ AAV
