Concentrate and purify lentivirus in a single unit operation
Achieve high functional recovery with minimal optimization.
IsoTag™ LV Reagent
Streamlined Lentiviral Vector Purification without Chromatography
IsoTag™ LV is a specialized reagent, engineered for the challenging requirements of small and large-scale manufacturing. It enables a robust, efficient, and consistent downstream purification process for VSV-G pseudotyped lentiviral feedstreams. Purification with IsoTag™ LV does not require the use of chromatography processes nor column packing, and is designed to reduce the costs and challenges associated with scaling from R&D to commercial production.
Affinity Liquid Phase Separation (ALPS):
Novel process to deliver transformational LV purification
The binding module is a custom-designed affinity ligand for VSV-G, allowing for exquisite specificity during purification. The phase separation module is used to enable highly specific sequestration of target biologics in a complex cellular environment. The ALPS process results in high yields and product quality without size target limitations. Read More about the ALPS-TFF process in our resources section →
+ Yield
>90% capture and ~40% functional recovery with adherent feedstream
+ Purity
>3 LRV of contaminating host cell proteins and DNA
+ Stability
Improved functional recovery in a variety of harsh conditions
+ Predictability
Binding efficacy observed with small-scale capture tests is predictive of larger scale purification.
+ Speed
Go from clarified harvest to purified lentivirus vector in the correct buffer in under 8 hours with a single unit operation.
+ Scalability
The affinity liquid phase separation TFF process (ALPS-TFF) scales linearly by increasing filter surface area as harvest volume increases.
IsoTag™ LV Reagent
IsoTag™ LV Reagent is currently available in 5mL vials. Using only 10mL (2 x 5mL) of IsoTag™ LV can purify up to 250mL of clarified LV harvest material in a TFF process. This product is currently limited to customers in the US and Canada. For international shipping outside of North America, please contact us.
IsoTag™ LV Webinar: Achieving high yields and purity with our chromatography-free process
Associate Director of R&D for Isolere Bio, Nikki Votaw, PhD, on innovative approaches to lentiviral vector purification, achieving high yields and purity with a chromatography-free process. View Webinar→