Our Technology
Phase separation evolved in cells to perform highly specific separations in complex environment.
We develop reagents that combine engineered biopolymers (with tunable phase separation) and affinity ligands that confer the unique material properties to the biologic target of interest. These reagents are then paired with high performing filtration processes that can improve productivity and quality. The linear scalability of these processes vertically integrate a biologic’s development as its batch size increases 1000-fold from the bench to the clinic to a commercial enterprise.
Our Reagents
The IsoTag™ reagent technology comprises simple recombinant proteins and straightforward processes that can plug into existing purification equipment along with validated, off-the-shelf consumables. These reagents comprise two key features: a phase separation module and an affinity binding module that is precisely engineered for the target biologic of interest. IsoTag™ reagents have been designed for diverse targets, including enveloped and non-enveloped viruses, antibodies, and nucleic acids.
Binding Module: Designed with exquisite specificity to rapidly bind the target in-solution
Robust, easy-to-express, stable ligands
Libraries of scaffolds with >10E9 diversity enable rapid ligand identification for for new applications
In vitro screening procedures provide high throughput, real-world binding data that can quickly identify novel binding properties or ideal reagent features (e.g. gentle elution conditions, target specificity, NaOH tolerance, etc.)
Can be multimerized as needed to achieve capacity, affinity, and avidity requirements
Phase Separation Module: pulls captured target into large, pure droplets on command
Custom designed at the sequence for each intended use case to provide precise performance
Transduce a variety of triggerable environmental stimuli into specific separation (e.g. temperature, salt conditions, pH).
Families of orthogonal modules to enable the next iteration on the technology’s bioprocessing capabilities
We are proud to collaborate with:
