Three Presenters at ACS-BIOT Conference, March 2024.
We are excited to have all our submitted presentations and posters accepted! If attending the American Chemical Society come see us at ACS-BIOT:
Rapid, high throughput AAV purification method using IsoTag™ AAV and low-speed centrifugation.
Jennifer Haley, Isolere Bio presenter
ACS-BIO Conference: March 17, 2024, 3:40 pm-4:00 pm
Adeno-associated virus (AAV) is a popular mechanism for gene therapy delivery due to the favorable safety profiles and tissue tropism. Advances in capsid engineering to more efficiently target tissues of interest have risen precipitously in recent years with the growth of library generation and screening AI tools. The increase in capsid libraries creates a demand for improved high-throughput screening methods. While advances have been made in scaled-down upstream manufacturing techniques and high throughput analytical techniques, there is still an industry need for high throughput downstream methods to sufficiently purify harvest material for analytics. IsoTag™ AAV offers a small-scale feed stream-independent method to purify AAV harvest in less than 2 hours without the use of resin or ultracentrifugation. This rapid AAV separation method has the potential to significantly streamline the purification of small-scale AAV production. Come learn more (or Contact Us).
Authors: Jennifer Haley, Jarrett Dobbins, Kimberly Anderson, Michael Dzuricky, Kelli Luginbuhl
If you can’t attend and want to learn more: AAV Research Grade out now.
Capturing ssRNA with recombinant phase separating biopolymers
James McGuinness, Isolere Bio presenter
ACS-BIO Conference: Tuesday, March 19, 2024, 7:00 PM - 9:00 PM
Poster, In-person.
The detection of in vitro transcription process-related impurities including dsRNA and DNA by nucleic-acid sensors produces an immune response that impairs the efficacy of mRNA therapeutics and presents a safety risk to patients. Current chromatographic purification modalities are costly, substantially reduce yield, and are prone to fouling, relegating them to polishing steps. Isolere Bio has developed Isotag™ RNA, which incorporates an RNA-binding motif specific to single-stranded RNA (ssRNA). The reagent was found to successfully capture RNA molecules in phase-separated droplets with at least 95% of all ssRNA collected from raw input samples while capturing less than 5% of linearized plasmid DNA and double-stranded RNA (dsRNA). Unlike column chromatography, we observe consistent binding capacity regardless of mRNA size; an especially important attribute for larger mRNAs and circular mRNA constructs. Learn more about a new purification of RNA.
If you can’t attend and want to learn more contact us.
Exceptional lentiviral recovery and purity with IsoTag™ LV: a novel, chromatography-free affinity reagent.
Nikki Votaw, Isolere Bio presenter
ACS-BIO Conference: Wednesday, March 20, 2024, 2:40 PM - 3:00 PM
We have developed a chromatography-free purification platform using our novel affinity reagent, IsoTag™ LV reagent, to streamline downstream purification into a two-step TFF process, completely eliminating chromatography. The IsoTag™ LV technology is a revolutionary leap forward in LV downstream purification and successful implementation of this platform could significantly reduce the volumetric burden placed on lentiviral manufacturing, accelerate clinical development, and reduce the cost burden on patients.
If you can’t attend and want to learn more: LV Prototype and Research grades coming in 2024.
