Better HCP Reduction with IsoTag: LentiVirus

IsoTag™LV and a comparison PEG-based lentiviral concentrating product were added to 1 mL samples of lentiviral harvest material and concentrated following manufacturers instructions. Pellets were resuspended in 1 mL PBS buffer and host cell protein was measured using an HCP ELISA kit (Cygnus #F650S), noting that lentiviral particles in the suspension create a low level of HCP in all groups. Using IsoTag™LV, 75% or more of the host cell proteins were removed, compared to about 30% from the comparison product.

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